Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Low Doses of Celecoxib Attenuate Gut Barrier Failure During Experimental Peritonitis

doi: 10.1038/labinvest.2013.119

Figure Lengend Snippet: (a) H&E-stained sections of ileum from mice 16 h after injection with NS, injection with 40 mg/kg LPS, sham operation, or CLP. Note abundant epithelial sloughing in animals injected with LPS or subjected to CLP. Data are representative of at least 9 animals in each group. (b) Transepithelial passage of FITC-dextran and bacteria during peritonitis. Mice were gavaged with FITC-dextran and E. coli 35354T prior to indicated treatments. Serum concentrations of FITC-dextran (top) and counts of viable E. coli in combined mesenteric lymph nodes and spleens (bottom) were determined 16 h post treatment. *, Significant differences (p ≤ 0.01, n ≥ 12 in each group). (c) Localization of JAM-A (red) in ileal epithelium 16 h after indicated treatments. Note increased re-distribution of JAM-A from borders to intracellular space of enterocytes in LPS and CLP samples. (d) Localization of ZO-1 (red) in ileal epithelium 16 h after indicated treatments. Note increased redistribution of ZO-1 from TJ to intracellular space of enterocytes in LPS and CLP samples. DAPI-stained nuclei appear in blue. Bar=50 μm. All images are representative of at least 3 animals in each group.

Article Snippet: Commensal E. coli strain 35354T was obtained from ATCC (Manassas, VA) and transformed with the pUC18 plasmid to confer ampicillin resistance.

Techniques: Staining, Injection, Bacteria

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Low Doses of Celecoxib Attenuate Gut Barrier Failure During Experimental Peritonitis

doi: 10.1038/labinvest.2013.119

Figure Lengend Snippet: Mice were gavaged with the test mixture of FITC-dextran and E. coli prior to experiments. Celecoxib at indicated doses (mg/kg) or vehicle were added to the test mixture. (a) Serum FITC-dextran levels 16 h after injection with NS or LPS, with or without various doses of Celecoxib, as indicated. (b) Serum FITC-dextran levels 16 h after sham operation or CLP, with or without various doses of Celecoxib, as indicated. (c) E. coli counts in lymphatic organs 16 h after injection with NS or LPS, with or without various doses of Celecoxib, as indicated. (d) E. coli counts in lymphatic organs 16 h after sham operation or CLP, with or without Celecoxib, as indicated. (e) E. coli counts in liver 16 h after indicated treatments, with or without 0.5 mg/kg Celecoxib, as indicated. Data are average ± SEM, n≥12 in each group. Significant differences: *, p ≤0.01 vs. LPS or CLP alone; **, p≤0.02 vs. LPS or CLP alone; #, p≤0.02 vs. NS or sham.

Article Snippet: Commensal E. coli strain 35354T was obtained from ATCC (Manassas, VA) and transformed with the pUC18 plasmid to confer ampicillin resistance.

Techniques: Injection

Journal: Molecular Plant Pathology

Article Title: Plant phenolic acids affect the virulence of P ectobacterium aroidearum and P . carotovorum ssp. brasiliense via quorum sensing regulation

doi: 10.1111/mpp.12295

Figure Lengend Snippet: Effects of cinnamic acid (CA; 0.25 mg/mL) and salicylic acid (SA; 0.21 mg/mL) on the production of quorum sensing (QS) signalling molecules by P ectobacterium aroidearum PC1 and P ectobacterium carotovorum ssp. brasiliense Pcb1692. (A) Purple colour exhibited by CV026, as response to N‐acyl‐homoserine lactones (AHLs) produced by PC1 (top) or Pcb1692 (bottom) that were grown with (control) and without non‐lethal concentrations of CA or SA. (B) Intensity of luminescence produced by E scherichia coli pSB401 induced by supernatants of strains PC1 (top) and Pcb1692 (bottom) grown with or without non‐lethal concentrations of CA or SA. Luminescence (250 ms) and absorbance (600 nm) were measured every 30 min for 14 h, and the relative luminescence (RLU = LU/OD 600 nm) was calculated. Blank was E . coli pSB401 grown in the absence of supernatants of Pectobacterium strains, and also E . coli pSB401 was supplemented with exogenous N‐(β‐ketocaproyl)‐l‐homoserine lactone (eAHL) at 100 nm. Each data point represents the mean ± standard error (SE) of eight replicates per treatment of one experiment, representative of two independent experiments with similar results. OD, optical density; LU, light units; RLU, relative light units.

Article Snippet: Table 2 Strain Description Reference/source Pectobacterium aroidearum PC1 Monocot strain isolated from Ornithogalum dubium , NCBI Accession no. PRJNA31289 Yishay et al . ( 2008 ) Pectobacterium carotovorum ssp. brasiliense Pcb1692 Dicot strain isolated from Solanum tuberosum , NCBI Accession no. PRJNA31121 Yishay et al . ( 2008 ) Chromobacterim violaceum CV026 Mini‐Tn5 mutant derived from C. violaceum ATCC 31532 Hg R , cvil ::Tn5 xyl E, Kan R , plus spontaneous Str R . AHL (C 4 –C 8 ) biosensor, produces violacein (pigment) only in the presence of eAHL McClean et al . ( 1997 ) Escherichia coli pSB401 luxRluxl ′ ( Photobacterium fischeri [ATCC 7744]):: lux CDABE ( Photorhabdus luminescens [ATCC 29999]) fusion; pACYC184‐derived, Tet R , AHL bioluminescent biosensor Winson et al . ( 1998 ) Open in a separate window AHL, N ‐acyl‐homoserine lactone; eAHL, N ‐(β‐ketocaproyl)‐ l ‐homoserine lactone; Hg R , hygromycin‐resistant; Kan R , kanamycin‐resistant; NCBI, National Center for Biotechnology Information; Str R , streptomycin‐resistant; Tet R , tetracycline‐resistant.

Techniques: Produced, Control

Journal: Molecular Plant Pathology

Article Title: Plant phenolic acids affect the virulence of P ectobacterium aroidearum and P . carotovorum ssp. brasiliense via quorum sensing regulation

doi: 10.1111/mpp.12295

Figure Lengend Snippet: Strains used in this study

Article Snippet: Table 2 Strain Description Reference/source Pectobacterium aroidearum PC1 Monocot strain isolated from Ornithogalum dubium , NCBI Accession no. PRJNA31289 Yishay et al . ( 2008 ) Pectobacterium carotovorum ssp. brasiliense Pcb1692 Dicot strain isolated from Solanum tuberosum , NCBI Accession no. PRJNA31121 Yishay et al . ( 2008 ) Chromobacterim violaceum CV026 Mini‐Tn5 mutant derived from C. violaceum ATCC 31532 Hg R , cvil ::Tn5 xyl E, Kan R , plus spontaneous Str R . AHL (C 4 –C 8 ) biosensor, produces violacein (pigment) only in the presence of eAHL McClean et al . ( 1997 ) Escherichia coli pSB401 luxRluxl ′ ( Photobacterium fischeri [ATCC 7744]):: lux CDABE ( Photorhabdus luminescens [ATCC 29999]) fusion; pACYC184‐derived, Tet R , AHL bioluminescent biosensor Winson et al . ( 1998 ) Open in a separate window AHL, N ‐acyl‐homoserine lactone; eAHL, N ‐(β‐ketocaproyl)‐ l ‐homoserine lactone; Hg R , hygromycin‐resistant; Kan R , kanamycin‐resistant; NCBI, National Center for Biotechnology Information; Str R , streptomycin‐resistant; Tet R , tetracycline‐resistant.

Techniques: Isolation, Mutagenesis, Derivative Assay